Primary murine hepatocytes exposed to fatty acids analyzed by Raman and infrared microscopy

Aim of this work is to present the phenotype and biochemical characteristics primary freshly isolated murine hepatocytes exposed oleic palmitic acids, most abundant fatty acids (FAs) in human diet serum. Excess FAs one factors that contributes non-alcoholic liver disease (NAFLD). The hallmark NAFLD overaccumulation lipids hepatic tissue formation lipid droplets (LDs). With time can progress into more advanced stages leading injury. Hepatocytes as cells are strongly involved process LDs formation, therefore widely used research on molecular mechanism development progression. Primary rarely investigations, due multistep isolation loss functionality under culture conditions. However, they offer substantial advantages, including preserved functions. In order investigate their content, Raman FTIR imaging were employed, provide spatial vibrational information studied label-free manner.